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Hektoen Enteric Agar

ARTICLE NO. OLFC-780
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For the differential & selective isolation of Salmonella and Shigella spp. from pathological specimens


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Light purple beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Green with yellowish ting colored opalescent
pH 7.5 ±0.2 at 25 ˚C (75.66 g/ l)
Solidification 40˚C
Color Green with yellowish ting colored opalescent gel

Contents

Ingredients Gram/Litre
Protease Peptone12.00
Yeast Extract3.00
Bile Salt Mixture9.00
Lactose12.00
Sucrose12.00
Salicin2.00
Sodium Chloride5.00
Sodium Thiosulfate5.00
Ferric Ammonium Citrate1.50
Acid Fuchsin0.10
Bromothymol Blue0.065
Agar14.00
pH at 25˚C7.5±0.2


Directions

Dissolve 75.66 grams in 1000 ml distilled water. Boil to dissolve the medium completely and Do not autoclave the medium, cool it to 42-45 °C and distribute aseptically. Ensure complete solidification inoculate test sample aseptically.