For differentiation of Staphylococci from Micrococci by anaerobic fermentation of glucose.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige to bluish green colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Purple colored opalescent solution
pH
7.4 ±0.2 at 25˚C (45.52 g/ l)
Solidification
Strength 0.3% agar gel
Color
Purple colored, clear to slightly opalescent gel forms in tubes as butts
Contents
Ingredients
Gram/Litre
Peptone
2.000
Yeast extract
0.500
Sodium Chloride
30.000
Glucose
10.000
Bromocresol purple
0.015
Agar
3.000
pH at 25˚C
7.4±0.2
Directions
Dissolve 45.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in test tubes. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 20
min, cool it to 42-45 °C and place it in upright position. Ensure solidification and inoculate test
sample aseptically.
