For the detecting aerobic and anaerobic breakdown of glucose.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige to bluish green colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Greenish blue colored opalescent solution
pH
7.1 ±0.2 at 25˚C (20.50 g/ l)
Solidification
Strength 0.3% agar gel
Color
Greenish blue colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
2.000
Sodium Chloride
5.000
Dipotassium phosphate
0.300
Glucose
10.000
Bromothymol blue
0.030
Agar
3.000
pH at 25˚C
7.1±0.2
Directions
Dissolve 20.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in test tubes. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 20
min, cool it to 42-45 °C and place it in upright position. Ensure solidification and inoculate test
sample aseptically.
