For isolation and enumeration of Lactobacillus from foods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Amber colored opalescent
pH
5.5 ±0.2 at 25˚C (84.75 g/l + 1.32 ml glacial acid)
Solidification
40˚C
Color
Amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
10.000
Yeast extract
5.000
Dextrose
20.000
Sodium acetate
25.000
Potassium phosphate
6.000
Ammonium citrate
2.000
Polysorbate 80
1.000
Magnesium sulphate
0.577
Manganese sulphate
0.120
Ferrous sulphate
0.034
Agar
15.000
pH at 25°C
5.5 ± 0.2
Directions
Dissolve 84.75 grams in 1000 ml distilled water containing 1.32 ml glacial acetic
acid, heat with constant stirring for 1-2 minutes to dissolve the medium completely. DO NOT
AUTOCLAVE. Always used freshly prepared medium. Mix well and distribute aseptically in
petri plates. Ensure complete solidification and inoculate test sample aseptically.
