For the differentiation of enteric organisms, especially Salmonella, based upon lysine
decarboxylation/ deamination and H2S production.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Purple colored opalescent
pH
6.7 ±0.2 at 25˚C (34.56 g/ l)
Solidification
40˚C
Color
Purple colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
5.000
Yeast extract
3.000
Dextrose
1.000
L-Lysine Hydrochloride
10.000
Ferric Ammonium citrate
0.500
Sodium thiosulfate
0.040
Bromocresol purple
0.020
Agar
15.000
pH at 25˚C
6.7 ±0.2
Directions
Dissolve 34.56 grams in 1000 ml distilled water. Boil to dissolve the medium
completely sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and
distribute aseptically in sterile test tubes. Kept in slight slanting position to form small slant with
large butt. Ensure complete solidification and inoculate test sample aseptically.
