For the cultivation of Corynebacterium diphtheriae from clinical samples and
confirmation of chromogenesis, proteolysis and the production of ascospores.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Basal: Light amber colored clear to slightly opalescent solution. After addition of horse serum: Off
pH
7.3 ±0.2 at 25 ˚C (8.75 g/ 250 ml + 750 ml sterile Horse Serum)
Contents
Ingredients
Gram/Litre
Special peptone
2.500
Meat Extract
2.500
Sodium Chloride
1.250
Dextrose
2.500
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 8.75 grams in 250 ml distilled water. If necessary, boil the medium to
dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115 °C) for 15 min,
cool it to 42-45 °C add 750 ml of sterile Horse Serum. Mix well and distribute aseptically in
desired. Sterilize the medium by inspissation at 80-85°C for 2 hours in free-flowing steam for at
least 3 consecutive days.
