For detection of presence and absence of coliform bacteria in water.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light greenish yellow colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Purple colored opalescent solution
pH
6.8 ±0.2 at 25˚C (30.50 g/ l)
Contents
Ingredients
Gram/Litre
Meat peptone
5.000
Tryptose
9.800
Meat extract
3.000
Lactose
7.460
Sodium chloride
2.460
Dipotassium phosphate
1.350
Monopotassium phosphate
1.350
Sodium lauryl sulphate
0.050
Bromocresol purple
0.0085
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 30.51 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute in desired. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically. To prepare triple strength medium,
dissolve 91.53 grams in 1000 ml distilled water.
