For presumptive identification of Clostridium perfringens in foods
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber, opalescent solution
pH
7.3 ±0.2 at 25 ˚C (50.50 g/ l)
Contents
Ingredients
Gram/Litre
Tryptone
15.000
Soya peptone
5.000
Yeast extract
5.000
Liver extract
7.000
Ferric ammonium citrate
1.000
Sodium metabisulphite
1.000
Tris
1.500
Agar
15.000
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 50.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and
add 100 mg of sodium sulphadiazine and 10000 IU of polymyxin B sulphate and 0.5 mg of
oleandomycin phosphate. Mix well and distribute aseptically desired and inoculate test sample
aseptically.
