For selective isolation and cultivation of Enterococci.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light greenish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Bluish Yellow colored opalescent Solution
pH
7.1 ±0.2 at 25 ˚C (63.00 g/ l)
Solidification
40˚C
Color
Bluish yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
17.000
Peptone
3.000
Yeast Extract
5.000
Bile Salt
10.000
Sodium Chloride
5.000
Sodium Citrate
1.000
Esculin
1.000
Ferric Ammonium Citrate
0.500
Sodium Azide
0.200
Agar
15.000
pH at 25˚C
7.1 ±0.2
Directions
Dissolve 58.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min.; cool it to 42-45 °C and
distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.