For Sucrose fermentation studies of microorganisms.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light Beige to pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Red color, clear slightly opalescent solution
pH
7.4 ±0.2 at 25℃ (21.00 g/ l)
Contents
Ingredients
Gram/Litre
Proteose peptone
10.000
Meat Extract
1.000
Sodium Chloride
5.000
Sucrose
5.000
Phenol red
0.018
pH at 25˚C
7.4 ±0.2
Directions
Dissolved 21.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute aseptically in test tubes containing inverted Durham’s tubes. Sterilize
by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C. Aseptically add filter
sterilized or autoclave sterilized carbohydrate solution to sterile basal medium. Mix well and
inoculate test sample aseptically.
