For differentiation of Proteus & Providencia from other members of Enterobacteriaceae
based on their ability to form phenyl pyruvic acid from phenylalanine.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.3 ±0.2 at 25 ˚C (23.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Yeast Extract
3.00
Dipotassium Phosphate
1.00
Sodium Chloride
5.00
Dl-Phenylalanine
2.00
Agar
15.00
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 26.00 grams in 1000 ml distilled water, boil to dissolve the medium
completely and distribute aseptically in test tubes. Sterilize by autoclaving at 15 lbs. pressure
(121 °C) for 15 min, cool it to 42-45 °C and allow the medium to solidify in a slanting position.
Ensure complete solidification and inoculate test sample aseptically.
