For the enumeration of viable bacteria in milk and dairy products by pour plate technique
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.0 ±0.2 at 25˚C (24.0 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
5.00
Yeast Extract
2.50
Glucose
1.00
Agar
15.00
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 23.5 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
