PNY Medium

ARTICLE NO. OLFC-1470
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For cultivation and isolation of Lactobacillus species.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Pale yellow colored opalescent solution
pH 6.0 ±0.2 at 25˚C (31.28 g/ l)
Solidification 40˚C
Color Pale yellow colored opalescent gel

Contents

Ingredients Gram/Litre
Peptone5.000
Yeast Extract5.000
Dextrose5.000
Potassium dihydrogen phosphate0.500
Dipotassium hydrogen phosphate0.500
Magnesium sulphate0.250
Manganese sulphate0.010
Ferrous sulphate0.010
Sodium chloride0.010
Zinc sulphate0.001
Copper sulphate0.001
Cobalt sulphate0.001
Agar15.000
pH at 25˚C6.0 ± 0.2


Directions

Dissolve 31.28 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in desired. Ensure complete solidification and inoculate test sample aseptically.