For cultivation and isolation of Lactobacillus species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Pale yellow colored opalescent solution
pH
6.0 ±0.2 at 25˚C (31.28 g/ l)
Solidification
40˚C
Color
Pale yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
5.000
Yeast Extract
5.000
Dextrose
5.000
Potassium dihydrogen phosphate
0.500
Dipotassium hydrogen phosphate
0.500
Magnesium sulphate
0.250
Manganese sulphate
0.010
Ferrous sulphate
0.010
Sodium chloride
0.010
Zinc sulphate
0.001
Copper sulphate
0.001
Cobalt sulphate
0.001
Agar
15.000
pH at 25˚C
6.0 ± 0.2
Directions
Dissolve 31.28 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in desired. Ensure complete solidification and inoculate test sample
aseptically.