For the isolation and enumeration of yeasts & molds from dairy & other food products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
5.6 ±0.2 at 25℃ (39.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Infusion from Potatoes (200 gm)
4.000
Dextrose
20.00
Agar
15.00
pH at 25˚C
5.6 ±0.2
Directions
Dissolve 39.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs.
pressure (121 °C) for 15 min, cool it to 42-45 °C. Distribute aseptically in desired and allow to
solidify. Ensure complete solidification and inoculate test sample aseptically. For more selectivity
to fungi, reduce the pH of medium to 3.6 to inhibit bacterial growth. Dissolve 39.00 grams in
1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to
45-50 °C. Add 10% sterile tartaric acid (approximately 1ml) till pH reaches to 3.6, mix well and
distribute in desired (avoid reheating the medium). Ensure complete solidification and inoculate
test sample.
