For the selective isolation & enumeration of yeasts & moulds from dairy & other food
products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
5.6 ±0.2 at 25 ˚C (40.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Potatoes, Infusion form
200.00
Dextrose
20.00
Chloramphenicol
0.050
Agar
15.00
pH at 25˚C
5.6 ±0.2
Directions
Dissolve 40.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs.
pressure (121 °C) for 15 min, cool it to 42-45 °C. Distribute aseptically in desired and allow to
solidify. Ensure complete solidification and inoculate test sample aseptically.
For more selectivity to fungi, reduce the pH of medium to 3.6 to inhibit bacterial growth.
Dissolve 40.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121
°C) for 15 min, cool it to 45-50 °C. Add 10 % sterile tartaric acid (approximately 1ml) till pH
reaches to 3.6, mix well and distribute in desired (avoid reheating the medium). Ensure complete
solidification and inoculate test sample.
