For the cultivation of Vibrio species from foods in accordance with APHA.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light yellow colored opalescent medium
pH
9.0 ±0.2 at 25 ˚C (50.00 g/ l)
Solidification
40˚C
Color
Light yellow colored Clear to slightly opalescent gel
Contents
Ingredients
Gram/Litre
Proteose peptone
15.000
Yeast extract
7.500
Casein acid hydrolysate
5.000
Starch soluble
1.000
Dipotassium hydrogen phosphate
5.000
Ammonium sulphate
1.500
Agar
15.000
pH at 25˚C
9.0 ±0.2
Directions
Dissolve 50.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in test tubes or petri plates. Allow to solidify and inoculate test sample
aseptically.
