For the detection of fluorescein production by Pseudomonas species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.0 ±0.2 at 25 ˚C (38.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
10.00
Proteose Peptone
10.00
Dipotassium Phosphate
1.50
Magnesium Sulfate
1.50
Agar
15.00
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 38.00 grams in 1000 ml distilled water contain 1% glycerol. Boil to dissolve
the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it
to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate
test sample aseptically.
