Rose Bengal Broth Base

ARTICLE NO. OLFC-1590
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For cultivation and enumeration of yeast and molds.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Pinkish beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Pink colored opalescent
pH 6.0 ±0.2 at 25 ˚C (16.50 g/ l)
Color Pink colored opalescent solution

Contents

Ingredients Gram/Litre
Soya Peptone5.000
Dextrose10.000
Monopotassium Phosphate1.000
Magnesium Sulfate0.500
Rose Bengal0.035
pH at 25˚C6.0 ±0.2


Directions

Dissolve 16.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically, if desired and inoculate test sample aseptically. Note: For more selectivity aseptically add 2 ml of chloramphenicol solution (0.1 g in 2ml of ethanol) in cooled broth base mix thoroughly and dispense in sterile test tube.