For the demonstration of rhamnose fermentation by Listeria monocytogenes.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Purple colored opalescent
pH
6.8 ±0.2 at 25˚C (16 .00 g/ l)
Contents
Ingredients
Gram/Litre
Meat Peptone
10.00
Meat Extract
1.00
Sodium Chloride
5.00
Bromocresol Purple
0.020
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 16.00 grams in 990 ml distilled water. Boil to dissolve the medium
completely and distribute 9.0 ml of media in test tubes containing inverted Durham’s tube.
Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and aseptically
add 1.0 ml of filter sterilized rhamnose sugar (the final concentration of rhamnose is maintained
at 1%), mix well and inoculate test sample.
