For enrichment of Salmonellae, based on its ability to multiply selectively at high osmotic
pressure, low pH and at 43°C, with modest nutritional requirements.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Pale yellow with blue tinge colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Greenish blue colored opalescent solution
pH
5.2 ±0.2 at 25 ˚C (49.17 g/ l)
Contents
Ingredients
Gram/Litre
Soya Peptone
4.500
Magnesium Chloride
36.000
Sodium Chloride
7.200
Potassium Dihydrogen Phosphate
1.440
Malachite Green
0.036
pH at 25˚C
5.2 ±0.2
Directions
Dissolve 49.17 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs
pressure (115°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
