For the selection, isolation and cultivation of yeasts and fungi.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber, opalescent solution
pH
7.3 ±0.2 at 25˚C (40.00 g/ l)
Solidification
40˚C
Color
Light amber, clear opalescent gel
Contents
Ingredients
Gram/Litre
Meat and Casein Peptone
10.000
Dextrose Monohydrate
40.000
Agar
15.000
pH at 25˚C
5.6 ±0.2
Directions
Dissolve 65.0 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs.
pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in to the petri plates
and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.
As per IP and EP the media can be fortified with antibiotic like 0.1g of benzylpenicillin sodium
and 0.1g of tetracycline per liter as sterile solution or alternatively 50 mg of chloramphenicol per
liter of medium before sterilization to achieve more selectivity.
