For isolation of Salmonella species from faeces, urine and other pathological materials.
Specifications
PHYSICAL PARAMETERS
Description
Part A and B fine, free flowing, powder.
Color
Part A: Light tan colored Part B: Off white
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent solution
pH
7.0 ±0.2 at 25˚C (Part A 19.00 g/ l & Part B 4.0g/l)
Contents
Ingredients
Gram/Litre
Part A
Tryptone
5.000
Lactose
4.000
Sodium Phosphate
10.000
Part B
Sodium Selenite
4.000
Final pH at 25˚C
7.0±0.2
Directions
Dissolve 19.0 grams of part A in 800 ml distilled water, add 4.0 g of part B in 200 ml
DW, mix Part A and B solutions, heat to dissolve the medium completely and distribute
aseptically in sterile test tubes. Sterilize in a boiling water bath for 10 minutes. DO NOT
AUTOCLAVE. Excessive heating is detrimental. Discard the prepared medium if large amount
red precipitate is settled at bottom which indicate the selenite reduction, if not then allow to cool
and inoculate test sample aseptically.
