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Sheep Blood Agar Base w/o Blood

ARTICLE NO. OLFC-1655
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For improved haemolytic reactions of organisms.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Dark amber, slightly opalescent
pH 7.4 ±0.2 at 25℃ (40.50 g/ l)
Solidification 40˚C
Color Without blood: dark amber, slightly opalescent gel. With 5% blood: cherry red, opaque gel.

Contents

Ingredients Gram/Litre
Tryptone14.000
Peptone4.500
Yeast Extract4.500
Sodium Chloride5.000
Agar12.500
pH at 25˚C7.4 ±0.2


Directions

Dissolve 40.5 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C, and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile petri plates. Ensure complete solidification and inoculate test sample aseptically.