For the differentiation of Enterobacteriaceae based on citrate utilization.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light yellow colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Forest green colored slightly opalescent
pH
6.8 ±0.1 at 25 ˚C (24.28 g/ l)
Solidification
40˚C
Color
Forest green colored slightly opalescent gel
Contents
Ingredients
Gram/Litre
Magnesium Sulphate
0.200
Ammonium Dihydrogen Phosphate
1.000
Dipotassium Phosphate
1.000
Sodium Citrate
2.000
Sodium Chloride
5.000
Bromothymol Blue
0.080
Agar
15.000
pH at 25˚C
6.8 ± 0.1
Directions
Dissolve 24.28 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in test tubes. Slant the tubes to have a small butt and slant, allow to
solidify. Ensure complete solidification and inoculate test sample aseptically.
