For differentiation of bacteria on the basis of indole production.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.2 ±0.2 at 25 ˚C (16.00 g/ l)
Contents
Ingredients
Gram/Litre
Special peptone
10.00
Tryptophan
1.00
Sodium Chloride
5.00
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 16.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically.
