For isolation and cultivation of Listeria from Henry’s light
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.3±0.2 at 25 ˚C (36.00 g/ l)
Contents
Ingredients
Gram/Litre
Tryptone
17.000
Soya peptone
3.000
Sodium chloride
5.000
Dipotassium phosphate
2.500
Dextrose
2.500
Yeast Extract
6.000
pH at 25˚C
7.3±0.2
Directions
Dissolve 36.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and inoculate test sample aseptically.
