For the cultivation and isolation of Thiobacillus species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored clear to slightly opalescent solution (turns red due to aeration on standing)
pH
7.1 ± 0.2 at 25˚C (49.00 g/ l)
Solidification
40˚C
Color
Light amber colored clear to slightly opalescent gel (turns red due to aeration on standing)
Contents
Ingredients
Gram/Litre
Ammonium Sulphate
0.400
Monopotassium Phosphate
4.000
Calcium Chloride
0.250
Ferrous Sulphate
0.010
Magnesium Sulphate
0.500
Sodium Thiosulphate
5.000
Agar
12.500
Directions
Dissolve 22.66 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C
and inoculate test sample aseptically.