TCBS Agar, Modified

ARTICLE NO. OLFC-1751
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For selective isolation of Vibrio cholerae and other enteropathogenic Vibrio's.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Light tan with greenish ting colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Greenish blue colored opalescent solution
pH 8.6 ± 0.1 at 25 ˚ C (88.08 g/ l)
Solidification 40˚C
Color Greenish blue colored opalescent gel

Contents

Ingredients Gram/Litre
Yeast extract5.00
Special peptone10.00
Sodium citrate10.00
Sodium thiosulfate10.00
Sodium chloride10.00
Sodium cholate3.00
Oxgall5.00
Sucrose20.00
Ferric citrate1.00
Bromothymol blue0.04
Thymol blue0.04
Agar14.00
pH at 25˚C8.6 ±0.1


Directions

Dissolve 88.08 grams in 1000 ml distilled water. Boil to dissolve the medium completely DO NOT AUTOCLAVE, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically