For selective isolation of Vibrio cholerae and other enteropathogenic Vibrio's.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light tan with greenish ting colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Greenish blue colored opalescent solution
pH
8.6 ± 0.1 at 25 ˚ C (88.08 g/ l)
Solidification
40˚C
Color
Greenish blue colored opalescent gel
Contents
Ingredients
Gram/Litre
Yeast extract
5.00
Special peptone
10.00
Sodium citrate
10.00
Sodium thiosulfate
10.00
Sodium chloride
10.00
Sodium cholate
3.00
Oxgall
5.00
Sucrose
20.00
Ferric citrate
1.00
Bromothymol blue
0.04
Thymol blue
0.04
Agar
14.00
pH at 25˚C
8.6 ±0.1
Directions
Dissolve 88.08 grams in 1000 ml distilled water. Boil to dissolve the medium
completely DO NOT AUTOCLAVE, cool it to 42-45 °C and distribute aseptically in petri plates.
Ensure complete solidification and inoculate test sample aseptically