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TCBS Agar (Thiosulphate Citrate Bile Salts Sucrose Agar)

ARTICLE NO. OLFC-1750
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For selective isolation of Vibrio cholerae & other enteropathogenic Vibrios causing food poisoning.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Light tan with greenish ting colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Greenish blue colored opalescent solution
pH 8.6 ± 0.2 at 25 ˚C (89.00 g/ l)
Solidification 40˚C
Color Greenish blue colored opalescent gel

Contents

Ingredients Gram/Litre
Yeast Extract5.00
Proteose Peptone10.00
Sodium Citrate10.00
Sodium Thiosulfate10.00
Oxgall8.00
Saccharose20.00
Sodium Chloride10.00
Ferric Citrate1.00
Bromothymol Blue0.04
Thymol Blue0.04
Agar15.00
pH at 25˚C8.6 ±0.2


Directions

Dissolve 89.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely DO NOT AUTOCLAVE, cool it to 42-45°C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.